Associate Professor Guy N. L. Jameson
- Associate Professor Guy N. L. Jameson
- Biophysical Chemistry
- Tel: (03) 479 8028
- Location: Science II, 5c4
My overall research area is spectroscopic and kinetic investigations of biological systems and novel materials. I study materials ranging from coordination polymers, through nanoparticles to small inorganic complexes as well as biological samples such as proteins.
I am an expert in Mössbauer spectroscopy and have established through the MacDiarmid Institute the first low-temperature Mössbauer laboratory in New Zealand. This capability is crucial for the study of iron-containing biological systems and vital when characterizing the properties of magnetic materials.
My research into biological systems brings together my expertise in spectroscopy and kinetics of proteins and small molecules and aims to understand the chemical basis of disease. Through studying how enzymes work at the molecular level we can understand how enzyme malfunction contributes to disease progression.
My research into enzyme mechanisms involves molecular biology and protein purification as well as physical, chemical and kinetic characterization techniques. As my research is highly interdisciplinary I collaborate with researchers from a variety of departments including chemistry, biochemistry, anatomy, physiology and pathology.
Currently we are studying two enzymes; cysteine dioxygenase which has an important role in disease by controlling cysteine levels, and myeloperoxidase, an enzyme critically involved in killing bacteria and other pathogens. Our research into cysteine dioxygenase has led to the development of a new enzymatic assay able to follow both substrate and product simultaneously and the first Mössbauer characterization of the enzyme. Through stopped-flow kinetic investigations of myeloperoxidase we have proposed that urate is a physiological substrate for the enzyme and is linked to cardiovascular disease.
Prospective research students should contact me by email. The University of Otago provides contestable PhD scholarships that are available to both domestic and international students. See here for more information.
Research Group (Lab 101)
- Dr Egor Tchesnokov
- (Canadian Institutes of Health Research Postdoctoral Fellow)
- Research Topic: Kinetic and spectroscopic characterization of enzymatic catalysis
- Richard Souness
- (PhD Student)
- Research Topic: Spectroscopic and density functional theory study of the enzyme cysteine dioxygenase
- Matthias Fellner
- (PhD Student)
- Research Topic: Enzyme kinetics of cysteine dioxygenase
- Antonia Seidel
- (PhD Student)
- Research Topic: Stopped-flow studies on mammalian peroxidases: myeloperoxidase and lactoperoxidase
- Casey Davies
- (Research Assistant)
Previous Group Members
- Dr. Eleni Siakkou (PhD student; graduated 2011)
- Seino Jongkees (Honours student; graduated 2007)
- Graham Fairweather (exchange student)
- Malcolm Rutledge (Research Assistant)
- Maria Kanitz (Research Assistant)
- Ministry of Science and Innovation
- Lottery Health
- Canadian Institutes of Health Research
- TEC Bright Futures Doctoral Scholarship
Cheong S.; Ferguson P.; Hermans I.F.; Jameson, G.N.L.; Prabakar, S.; Herman, D.A.J.; Tilley, R.D.*, Synthesis and Stability of Highly Crystalline and Stable Iron/Iron Oxide Core/Shell Nanoparticles for Biomedical Applications, ChemPlusChem, 2012, 77 (2), 135-140.
Tchesnokov E.P.; Wilbanks S.M.; Jameson G.N.L.*, A Strongly Bound High-Spin Iron(II) Coordinates Cysteine and Homocysteine in Cysteine Dioxygenase, Biochemistry, 2012, 51 (1), 257-264.
Siakkou, E.; Rutledge, M.T.; Wilbanks, S.M.; Jameson, G.N.L.*, Correlating crosslink formation with enzymatic activity in cysteine dioxygenase, Biochim. Biophys. Acta, Proteins Proteomics, 2011, 1814 (12) 2003-2009.
Tidén, A-K.; Sjögren, T.; Svensson, M.; Bernlind, A.; Senthilmohan, R.; Auchère, F.; Norman, H.; Markgren, P-O.; Gustavsson, S.; Schmidt, S.; Lundquist, S.; Forbes, L.V.; Magon, N.J.; Jameson, G.N.L.; Eriksson, H.; Kettle, A.J.*, 2-Thioxanthines are mechanism-based inactivators of myeloperoxidase that block oxidative stress during inflammation, J. Biol. Chem. 2011, 286 (43) 37578-37589.
Kitchen, J.A.; White, N.G.; Jameson, G.N.L.; Tallon, J.L.; Brooker, S.*, Effect of Counteranion X on the Spin Crossover Properties of a Family of Diiron(II) Triazole Complexes [Fe2(II)(PMAT)2]X4, Inorg. Chem., 2011, 50 (10), 4586-4597.
Meotti, F.C.; Jameson, G.N.L.; Turner, R.; Harwood, D.T.; Stockwell, S.; Rees, M.D.; Thomas, S.R.; Kettle, A.J.* Urate as a physiological substrate for myeloperoxidase: implications for hyperuricemia and inflammation, J. Biol. Chem., 2011, 286 (15), 12901-12911. Jameson, G.N.L.*, Iron, cysteine and Parkinson's disease, Monatsh. Chem., 2011, 142 (4), 325-329.
Jameson, G.N.L.*, Iron, cysteine and Parkinson's disease, Monatsh. Chem., 2011, 142 (4), 325-329.
Olguin J.; Jameson, G.N.L.; Brooker, S.* Two dinuclear iron(II) complexes K[Fe2(L1)(SCN)4]2(C3H8O) and [Fe2(L1)(SeCN)(3)(C5H5N)]H2O are stabilised in the '[HS-LS]' state by a bis-tetradentate pyrazolate-based ligand, J. Chem. Soc. Dalton Trans., 2011, 40 (18), 5086-5089.
Archer R.J.; Hawes C.S.; Jameson G.N.L., Mckee, V., Moubaraki, B., Chilton, N.F., Murray, K.S., Schmitt, W., Kruger, P.E.*, Partial spin crossover behaviour in a dinuclear iron(II) triple helicate, J. Chem. Soc. Dalton Trans., 2011, 40 (45) 12368-12373.
Olguin, J.; Jameson, G.N.L.; Brooker, S.*, Two mononuclear iron(II) complexes of 4-phenylpyrazole-5-carbaldehyde derived ligands are stabilised in different spin states, RSC Advances, 2011, 1, 52-57.
Siakkou, E.; Wilbanks, S.M.W.; Jameson, G.N.L.* Simplified cysteine dioxygenase activity assay allows simultaneous quantitation of both substrate and product, Anal. Biochem., 2010, 405 (1), 127-131.
Kitchen, J.A.; Jameson, G.N.L.; Tallon, J.L.; Brooker, S.* Spin crossover in co-crystallised 2:1 cis trans [FeII(pldpt)2(NCS)2] occurs only in 1/3 of the iron centres, Chem. Commun., 2010, 46, 3200-3202.
Kitchen, J.A.; White, N. G.; Gandolfi, C.; Albrecht, M.; Jameson, G.N.L.; Tallon, J.L.; Brooker, S.* Room-temperature spin crossover and Langmuir-Blodgett film formation of an iron(II) triazole complex featuring a long alkyl chain substituent: the tail that wags the dog, Chem. Commun., 2010, 46 (35), 6464-6466.
Kitchen, J. A.; Jameson, G.N.L.; Milway, V. A.; Tallon, J.L.; Brooker, S.* When three is not a crowd: the first trinuclear complexes of N4-substituted-3,5-dipyridyl-1,2,4-triazole ligands, [FeII3(Rdpt)4(NCS)6], J. Chem. Soc. Dalton Trans., 2010, 39 (33) 7637-7639.
Nagy, P.*; Jameson, G. N. L.; Winterbourn, C. C. Kinetics and Mechanisms of the Reaction of Hypothiocyanous Acid with 5-Thio-2-nitrobenzoic Acid and Reduced Glutathione, Chem. Res. Toxicol., 2009, 22(11), 1833-1840.
Freinbichler, W.; Soliman, A.; Jameson, R.F.; Jameson, G.N.L.; Linert, W.* Kinetic study of the complex reaction between copper(II) and 2-(2-hydroxy-3-methoxyphenyl)benzothiazole, Spectrochim. Acta A, 2009, 74(1), 30-35.
Jameson, G.N.L.*; Werner, F.; Bartel, M.; Absmeier, A.; Reissner, M.; Kitchen, J.A.; Brooker, S.; Caneschi, A.; Carbonera, C.; Létard, J-F.; Linert, W.* Anion, Solvent and Time Dependence of High-Spin-Low-Spin Interactions in a 3D Coordination Polymer, Eur. J. Inorg. Chem., 2009, 26, 3948-3959.
Kleffmann, T.; Jongkees, S.A.K.; Fairweather, G.; Wilbanks, S.M.; Jameson, G.N.L.* Mass-spectrometric characterization of two posttranslational modifications of cysteine dioxygenase, J. Biol. Inorg. Chem. 2009, 14(6), 913-921.